孙前文 博士

副教授

2022-今        副教授，亚星221net 亚星221net

2014-2021    助理教授，亚星221net 亚星221net

2014-今        研究员, 清华-北大生命科学联合中心

2009-2014    博士后，John Innes Centre, Norwich, UK

2004-2009    博士 华中农业大学 生科院

2000-2004    学士 华中农业大学 生科院

●  研究兴趣、领域：

我们实验室感兴趣各个层面的基因组调控事件，近期研究重点：（1）一种新型染色质状态Transcription Resistive的形成与调控机制和生物学意义；（2）半自主细胞器的发育和发生机制。

 

●  已发表论文：

(*corresponding or co-corresponding authors)

13.     Yao Yao; Jincong Zhou; Jiacheng Wang; Xue Lei; Anjie Jiang; Qianwen Sun*. H3K36 Methylation Stamps Transcription Resistive to Preserve Development in Plants. Nature Plants, 2025. DOI : 10.1038/s41477-025-01962-6.

12.     Weifeng Zhang, Lingling Cheng, Kuan Li, Leiming Xie, Jinyao Ji, Xue Lei, Anjie Jiang, Chunlai Chen, Haitao Li, Pilong Li and Qianwen Sun*. Evolutional heterochromatin condensation delineates chromocenter formation and retrotransposon silencing in plants. Nature Plants. 2024. https://www.nature.com/articles/s41477-024-01746-4.

11.     Weifeng Zhang, Zhuo Yang, Wenjie Wang and Qianwen Sun*. Primase promotes the competition between transcription and replication on the same template strand resulting in DNA damage. Nature Communications. 2024; 15, 73. https://doi.org/10.1038/s41467-023-44443-0.

10.     Qin Li, Jincong Zhou, Shuai Li, Weifeng Zhang, Yingxue Du, Kuan Li, Yingxiang Wang and Qianwen Sun*. DNA polymerase ε harmonizes topological states and R-loops formation to maintain genome integrity in Arabidopsis. Nature Communications. 2023; 14, 7763. https://doi.org/10.1038/s41467-023-43680-7.

9.      Jincong Zhou, Xue Lei, Sarfraz Shafiq, Weifeng Zhang, Qin Li, Kuan Li, Jiafu Zhu, Zhicheng Dong, Xin-Jian He and Qianwen Sun*. DDM1-mediated R-loop resolution and H2A.Z exclusion facilitates heterochromatin formation in Arabidopsis. Science Advances. 2023 Aug;9(32):eadg2699. doi: 10.1126/sciadv.adg2699.

8.      Wei Xu*, Chao Liu, Zhe Zhang, Changbin Sun, Qin Li, Kuan Li, Hui Jiang*, Wei Li* and Qianwen Sun*. DEtail-seq is an ultra-efficient and convenient method for meiotic DNA break profiling in multiple organisms. Sci China Life Sci, 2023 June; 66(6): 1392–1407. https://doi.org/10.1007/s11427-022-2277-y.

7.      Kuan Li, Zhenzhen Wu, Jincong Zhou, Wei Xu, Ling Li, Chao Liu, Wei Li, Chengjun Zhang* and Qianwen Sun*. R-loopAtlas: An integrated R-loop resource from 254 plant species sustained by a deep-learning-based tool. Molecular Plant. 2023 Mar;16(3):493-496. doi: 10.1016/j.molp.2022.12.012.

6.       Kunpeng Liu and Qianwen Sun*. Intragenic tRNA-promoted R-loops orchestrate transcription interference for plant oxidative stress responses. The Plant Cell, 2021, 33: 3574–3591.

5.       Lingling Cheng, Wenjie Wang, Yao Yao and Qianwen Sun*. Mitochondrial RNase H1 activity regulates R-loop homeostasis to maintain genome integrity and enable early embryogenesis in Arabidopsis. PLOS Biology, 2021, 19(8): e3001357.

4.       Wenjie Wang, Kuan Li, Zhuo Yang, Quancan Hou, Wei W. Zhao and Qianwen Sun*. RNase H1C collaborates with ssDNA binding proteins WHY1/3 and recombinase RecA1 to fulfill the DNA damage repair in Arabidopsis chloroplasts. Nucleic Acids Research, 2021 Jul; 49(12): 6771–6787.

3.       Wei Xu, Kuan Li, Shuai Li, Quancan Hou, Yushun Zhang, Kunpeng Liu and Qianwen Sun*. The R-loop atlas of Arabidopsis development and responses to environmental stimuli. The Plant Cell, 2020 Apr; 32(4):888-903.

2.       Zhuo Yang, Quancan Hou, Lingling Cheng, Wei Xu, Yantao Hong, Shuai Li and Qianwen Sun*. RNase H1 Cooperates with DNA Gyrases to Restrict R-Loops and Maintain Genome Integrity in Arabidopsis Chloroplasts. The Plant Cell, 2017 Oct; 29(10):2478-2497.

1.       Wei Xu, Hui Xu, Kuan Li, Yingxu Fan, Yang Liu, Xuerui Yang* and Qianwen Sun*. The R-loop is a common chromatin feature of the Arabidopsis genome. Nature Plants, 2017 Sep; 3(9):704-714.

●  联系方式：

地址：亚星221net生物技术馆4段313室(办公室)，309室(实验室)

电话:  +86-10-62784002 (办公室); 62796360 (实验室)

电子邮件：sunqianwen(a t)mail.tsinghua.edu.cn