王宏伟 博士

教授、博士生导师

1992-1996     亚星221net生物科学与技术系，学士

1996-2001     亚星221net生物科学与技术系，博士

2001-2006     美国劳伦斯伯克利国家实验室生命科学部，博士后

2006-2008     美国劳伦斯伯克利国家实验室生命科学部，研究科学家

2009-2011     美国耶鲁大学分子生物物理与生物化学系，Tenure-Track助理教授

2010.12-至今 亚星221net，教授、博导

2016.04-2021.04   亚星221net院长

●  研究兴趣、领域：

利用冷冻电子显微学研究生物大分子复合体的结构与分子机理。主要研究方向如下：

1. 更高效、更高分辨率的冷冻电镜方法学开发；

2. 核酸质量控制的分子机制和调控；

3. 细胞骨架和生物膜系统的协调机制。

●  代表性论文：

1. Zheng, LM, Xu J, Wang W, Gao X, Zhao C, Guo W, Sun L, Cheng H, Meng F, Chen B, Sun W, Jia X, Zhou X, Wu K, Liu Z, Ding F, Liu N*, Wang HW*, Peng HL*. (2024) Self-assembled superstructure alleviates air-water interface effect in cryo-EM. Nat Commun., 15(1):7300.

2. Yang Z, Fan J, Wang J, Fan X, Ouyang Z*, Wang HW*, Zhou XY*. (2024) Electrospray-assisted cryo-EM sample preparation to mitigate interfacial effects. Nat Methods, 21(6):1023-1032.

3. Xu J, Gao XY, Zheng LM, Jia X, Xu K, Ma Y, Wei XD, Liu N*, Peng HL*, Wang HW*. (2024) Graphene sandwich-based biological specimen preparation for cryo-EM analysis. Proc Natl Acad. Sci USA, 121(5):e2309384121.

4. Zhou J, Wang A, Song Y, Liu N, Wang J, Li Y, Liang X, Li GH, Chu HY, Wang HW*. (2023) Structural insights into the mechanism of GTP initiation of microtubule assembly. Nat. Commun., 14(1):5980.

5. You X, Zhang X, Cheng J, Xiao YN, Ma JF, Sun S, Zhang XZ*, Wang HW*, Sui SF*. (2023) In situ structure of the red algal phycobilisome–PSII–PSI–LHC megacomplex. Nature, 616(7955):199-206.

6. Zheng LM, Liu N*, Gao XY, Zhu WQ, Liu K, Wu C, Yan R, Zhang JC, Gao X, Yao YT, Deng B, Xu J, Lu Y, Liu, ZM, Li MS, Wei XD*, Wang HW*, Peng HL*. (2023) Uniform thin ice on ultraflat graphene for high-resolution cryo-EM. Nat Methods, 20(1):123-130.

7. Lu Y, Liu N*, Liu Y, Zheng LM, Yang JH, Wang J, Jia X, Zi QR, Peng HL*, Rao Y*, Wang HW*. (2022) Functionalized graphene grids with various charges for single-particle cryo-EM. Nat. Commun., 13(1):6718.

8. Su S, Wang J, Deng T, Yuan X, He J, Liu N, Li XM, Huang Y, Wang HW*, Ma JB*. (2022) Structural insights into dsRNA processing by Drosophila Dicer-2-Loqs-PD. Nature, 607(7918):399-406.

9. Liu N, Zheng LM, Xu J, Wang J, Hu CX, Lan J, Zhang X, Zhang J, Xu K, Cheng H, Yang Z, Gao X, Wang X, Peng HL*, Chen YN*, Wang HW*. (2021) Reduced graphene oxide membrane as supporting film for high-resolution cryo-EM. Biophysics Reports, 7(3): 227-238. (Cover)

10. Jin W, Wang J, Liu CP, Wang HW*, Xu RM*. (2020) Structural Basis for pri-miRNA Recognition by Drosha. Mol. Cell, 78(3): 423-439.

11. Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J*, Wang HW*. (2019) Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution. Nat. Commun., 10(1):2386.

12. Liu N, Zhang J, Chen Y*, Liu C, Zhang X, Xu K, Wen J, Luo Z, Chen S, Gao P, Jia K, Liu Z, Peng H*, Wang HW*. (2019) Bioactive Functionalized Monolayer Graphene for High-Resolution Cryo-Electron Microscopy. J. Am. Chem. Soc., 141(9):4016-4025.

13. Wang J, Wang J, Hu M, Wu S, Qi J, Wang G, Han Z, Qi Y, Gao N, Wang HW*, Zhou JM*, Chai J*. (2019) Ligand-triggered allosteric ADP release primes a plant NLR complex. Science, 364(6435). pii: eaav5868.

14. Wang J, Hu M, Wang J, Qi J, Han Z, Wang G, Qi Y, Wang HW*, Zhou JM*, Chai J*. (2019) Reconstitution and structure of a plant NLR resistosome conferring immunity. Science, 364(6435). pii: eaav5870.

15. Liu Z, Wang J, Cheng H, Ke X, Sun L, Zhang Q, Wang HW*. (2018) Cryo-EM Structure of Human Dicer and Its Complexes with a Pre-miRNA Substrate. Cell, 173(5):1191-1203.

●  联系方式：

电话：+86-10-62772390 (lab)

         +86-10-62798651 (office)

E-mail: hongweiwang@tsinghua.edu.cn

Lab website: http://cryoem.life.tsinghua.edu.cn/